Insight into polyhydroxyalkanoate (PHA) production from xylose and extracellular PHA degradation by a thermophilic Schlegelella thermodepolymerans

Accumulation of non-degradable plastic waste in the environment might be prevented by use biodegradable polyhydroxyalkanoate (PHA). In this study, thermophile Schlegelella thermodepolymerans produced up to 80 wt% PHA based on dry cell mass. The largest granules were found cells within 48 h using 20 g/L xylose, a C/N ratio 100, an initial pH 7, at 50 °C. substrate consumption, changes, and growth monitored, revealing time dependency production S. thermodepolymerans. metabolic pathways from xylose identified proteomic analysis, involvement classic phaCAB, de novo fatty acid biosynthesis, β-oxidation. addition, it was shown that degraded extracellular with high efficiency